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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Semiárido. |
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Data corrente: |
10/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, A. S.; PEREIRA, L. G. R.; PEDREIRA, M. dos S.; MACHADO, F. S.; CAMPOS, M. M.; CORTINHAS C. S.; ACEDO, T. S.; SANTOS, R. D. dos; RODRIGUES, J. P. P.; MAURICIO, R. M.; TOMICH, T. R. |
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Afiliação: |
Abias Santos Silva; LUIZ GUSTAVO RIBEIRO PEREIRA, CNPGL; Márcio dos Santos Pedreira; FERNANDA SAMARINI MACHADO, CNPGL; MARIANA MAGALHAES CAMPOS, CNPGL; Cristina Simões Cortinhas; Tiago Sabella Acedo; RAFAEL DANTAS DOS SANTOS, CPATSA; João Paulo Pacheco Rodrigues; Rogério Martins Maurício; THIERRY RIBEIRO TOMICH, CNPGL. |
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Título: |
Effects of exogenous amylase on the in vitro digestion kinetics of whole-crop maize silages made from flint or dent grain type at different phenological stages grown in tropical condition. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Animal Physiololgy and Animal Nutrition, v. 104, n. 1, p. 76-87, 2020. |
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DOI: |
https://doi.org/10.1111/jpn.13234 |
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Idioma: |
Inglês |
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Conteúdo: |
The effect of exogenous amylase on the in vitro rumen digestion kinetics of wholecrop maize silage made from dent (RB9004) or flint grain type (RB9308) was evaluated at different phenological stages: soft dough (SOD), early dent (EAD), ½ milkline (½M) and ¾ milkline (¾M). Forage was harvested from 70 to 110 days after sowing. Two rumen-cannulated cows receiving or not exogenous amylase (0.7 g/kg dry matter-DM, provided to achieve 396 kilo Novo units of amylase activity/kg of TMR DM) were used as donor of ruminal fluid. The in vitro gas production kinetics was evaluated according to a dual?pool logistic model. The chemical composition and gas production kinetics were affected by the hybrid and phenological stages. The flint hybrid had lower range for chemical analysis among physiological stages. Harvesting at ½M and ¾M improved DM content, bromatological composition and silage quality parameters compared to dent or flint types. Amylase (i) increased methane (CH4) production and in vitro dry matter digestibility (IVDMD) in ½M stage, (ii) improved digestion kinetics by reducing lag time and increasing total gas production and fermentation rates of non?fibrous carbohydrates (NFC) and fibrous carbohydrates (FC), and (iii) increased extent and fermentation rate of NFC and increased fermentation rate of FC fraction in whole?crop maize silages produced from dent or flint types in all phenological stages. Harvesting between ½M and ¾M is the best phenological stage to improve chemical composition and silage quality parameters. Exogenous amylase showed improvements on fibre digestion of silages at ½M and ¾M phenological stages in both grain types of corn. MenosThe effect of exogenous amylase on the in vitro rumen digestion kinetics of wholecrop maize silage made from dent (RB9004) or flint grain type (RB9308) was evaluated at different phenological stages: soft dough (SOD), early dent (EAD), ½ milkline (½M) and ¾ milkline (¾M). Forage was harvested from 70 to 110 days after sowing. Two rumen-cannulated cows receiving or not exogenous amylase (0.7 g/kg dry matter-DM, provided to achieve 396 kilo Novo units of amylase activity/kg of TMR DM) were used as donor of ruminal fluid. The in vitro gas production kinetics was evaluated according to a dual?pool logistic model. The chemical composition and gas production kinetics were affected by the hybrid and phenological stages. The flint hybrid had lower range for chemical analysis among physiological stages. Harvesting at ½M and ¾M improved DM content, bromatological composition and silage quality parameters compared to dent or flint types. Amylase (i) increased methane (CH4) production and in vitro dry matter digestibility (IVDMD) in ½M stage, (ii) improved digestion kinetics by reducing lag time and increasing total gas production and fermentation rates of non?fibrous carbohydrates (NFC) and fibrous carbohydrates (FC), and (iii) increased extent and fermentation rate of NFC and increased fermentation rate of FC fraction in whole?crop maize silages produced from dent or flint types in all phenological stages. Harvesting between ½M and ¾M is the best phenological stage to improve chemical... Mostrar Tudo |
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Palavras-Chave: |
Carboidratos; Digestão in vitro; Tempo de colheita. |
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Thesagro: |
Digestibilidade; Enzima; Metano; Milho; Silagem. |
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Thesaurus Nal: |
Corn silage; Grass silage; Silage. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02879naa a2200385 a 4500
001 2118526
005 2024-02-06
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/jpn.13234$2DOI
100 1 $aSILVA, A. S.
245 $aEffects of exogenous amylase on the in vitro digestion kinetics of whole-crop maize silages made from flint or dent grain type at different phenological stages grown in tropical condition.$h[electronic resource]
260 $c2020
520 $aThe effect of exogenous amylase on the in vitro rumen digestion kinetics of wholecrop maize silage made from dent (RB9004) or flint grain type (RB9308) was evaluated at different phenological stages: soft dough (SOD), early dent (EAD), ½ milkline (½M) and ¾ milkline (¾M). Forage was harvested from 70 to 110 days after sowing. Two rumen-cannulated cows receiving or not exogenous amylase (0.7 g/kg dry matter-DM, provided to achieve 396 kilo Novo units of amylase activity/kg of TMR DM) were used as donor of ruminal fluid. The in vitro gas production kinetics was evaluated according to a dual?pool logistic model. The chemical composition and gas production kinetics were affected by the hybrid and phenological stages. The flint hybrid had lower range for chemical analysis among physiological stages. Harvesting at ½M and ¾M improved DM content, bromatological composition and silage quality parameters compared to dent or flint types. Amylase (i) increased methane (CH4) production and in vitro dry matter digestibility (IVDMD) in ½M stage, (ii) improved digestion kinetics by reducing lag time and increasing total gas production and fermentation rates of non?fibrous carbohydrates (NFC) and fibrous carbohydrates (FC), and (iii) increased extent and fermentation rate of NFC and increased fermentation rate of FC fraction in whole?crop maize silages produced from dent or flint types in all phenological stages. Harvesting between ½M and ¾M is the best phenological stage to improve chemical composition and silage quality parameters. Exogenous amylase showed improvements on fibre digestion of silages at ½M and ¾M phenological stages in both grain types of corn.
650 $aCorn silage
650 $aGrass silage
650 $aSilage
650 $aDigestibilidade
650 $aEnzima
650 $aMetano
650 $aMilho
650 $aSilagem
653 $aCarboidratos
653 $aDigestão in vitro
653 $aTempo de colheita
700 1 $aPEREIRA, L. G. R.
700 1 $aPEDREIRA, M. dos S.
700 1 $aMACHADO, F. S.
700 1 $aCAMPOS, M. M.
700 1 $aCORTINHAS C. S.
700 1 $aACEDO, T. S.
700 1 $aSANTOS, R. D. dos
700 1 $aRODRIGUES, J. P. P.
700 1 $aMAURICIO, R. M.
700 1 $aTOMICH, T. R.
773 $tJournal of Animal Physiololgy and Animal Nutrition$gv. 104, n. 1, p. 76-87, 2020.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
05/04/2021 |
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Data da última atualização: |
10/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
CÔNSOLO, N. R. B.; BUARQUE, V. L. M.; SILVA, J.; POLETI, M. D.; BARBOSA, L. C. G. S.; HIGUERA-PADILLA, A.; GOMEZ, J. F. M.; COLNAGO, L. A.; GERRARD, D. E.; SARAN NETO, A.; SILVA, S. L. |
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Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
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Título: |
Muscle and liver metabolomic signatures associated with residual feed intake in Nellore cattle. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Animal Feed Science and Technology, v. 271, 114757, 2021. |
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ISSN: |
0377-8401 |
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DOI: |
https://doi.org/10.1016/j.anifeedsci.2020.114757 |
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Idioma: |
Inglês |
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Conteúdo: |
Identification of those cattle genetics with superior feed efficiency is necessary to increase the productivity of the entire beef industry. The aim of this study was to investigate metabolites profiling in the liver and muscle to identify physiological changes in animals in the livestock system with low or high residual feed intake. Liver and muscle were collected after slaughter from Nellore males, with high and low residual feed intake (H-RFI, n = 10 and L-RFI, n = 10, respectively). Metabolites were extracted with cold methanol/water solution (4:3 v/v). Metabolomics analyses were performed by 1D 1 H nuclear magnetic resonance. Metabolites were identified using Chenomx and analysed using MetaboAnalyst 4.0. The residual feed intake classification did not change most of animal performance traits; however, animals with lower residual feed intake presented greater carcass dressing, lower carcass fat deposition. Sixty-three compounds were identified in the liver and 31 in muscle. Coexpression network analysis was carried out and identified four metabolite network modules correlated with the phenotypic traits, indicating common metabolites with a variate important projection score that influences residual feed intake. Metabolites in liver samples was correlated with energy and protein metabolism and on muscle with fat metabolism. Differences on energetic metabolism, including of carbohydrate- and fat-correlated compounds, according to residual feed intake can be assessed by importance of concentration of citrate, isocitrate, glucose-6-phosphate, nicotinamide adenine dinucleotide + hydrogen and creatine phosphate (in liver) and choline, glycine, glycerol, malonate, glucose-6- phosphate and 3-hydroxybutyrate (in muscle). Moreover, differences on protein metabolism, MenosIdentification of those cattle genetics with superior feed efficiency is necessary to increase the productivity of the entire beef industry. The aim of this study was to investigate metabolites profiling in the liver and muscle to identify physiological changes in animals in the livestock system with low or high residual feed intake. Liver and muscle were collected after slaughter from Nellore males, with high and low residual feed intake (H-RFI, n = 10 and L-RFI, n = 10, respectively). Metabolites were extracted with cold methanol/water solution (4:3 v/v). Metabolomics analyses were performed by 1D 1 H nuclear magnetic resonance. Metabolites were identified using Chenomx and analysed using MetaboAnalyst 4.0. The residual feed intake classification did not change most of animal performance traits; however, animals with lower residual feed intake presented greater carcass dressing, lower carcass fat deposition. Sixty-three compounds were identified in the liver and 31 in muscle. Coexpression network analysis was carried out and identified four metabolite network modules correlated with the phenotypic traits, indicating common metabolites with a variate important projection score that influences residual feed intake. Metabolites in liver samples was correlated with energy and protein metabolism and on muscle with fat metabolism. Differences on energetic metabolism, including of carbohydrate- and fat-correlated compounds, according to residual feed intake can be assessed by imp... Mostrar Tudo |
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Palavras-Chave: |
Muscle; Residual feed intake. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02667naa a2200289 a 4500
001 2131049
005 2022-06-10
008 2021 bl uuuu u00u1 u #d
022 $a0377-8401
024 7 $ahttps://doi.org/10.1016/j.anifeedsci.2020.114757$2DOI
100 1 $aCÔNSOLO, N. R. B.
245 $aMuscle and liver metabolomic signatures associated with residual feed intake in Nellore cattle.$h[electronic resource]
260 $c2021
520 $aIdentification of those cattle genetics with superior feed efficiency is necessary to increase the productivity of the entire beef industry. The aim of this study was to investigate metabolites profiling in the liver and muscle to identify physiological changes in animals in the livestock system with low or high residual feed intake. Liver and muscle were collected after slaughter from Nellore males, with high and low residual feed intake (H-RFI, n = 10 and L-RFI, n = 10, respectively). Metabolites were extracted with cold methanol/water solution (4:3 v/v). Metabolomics analyses were performed by 1D 1 H nuclear magnetic resonance. Metabolites were identified using Chenomx and analysed using MetaboAnalyst 4.0. The residual feed intake classification did not change most of animal performance traits; however, animals with lower residual feed intake presented greater carcass dressing, lower carcass fat deposition. Sixty-three compounds were identified in the liver and 31 in muscle. Coexpression network analysis was carried out and identified four metabolite network modules correlated with the phenotypic traits, indicating common metabolites with a variate important projection score that influences residual feed intake. Metabolites in liver samples was correlated with energy and protein metabolism and on muscle with fat metabolism. Differences on energetic metabolism, including of carbohydrate- and fat-correlated compounds, according to residual feed intake can be assessed by importance of concentration of citrate, isocitrate, glucose-6-phosphate, nicotinamide adenine dinucleotide + hydrogen and creatine phosphate (in liver) and choline, glycine, glycerol, malonate, glucose-6- phosphate and 3-hydroxybutyrate (in muscle). Moreover, differences on protein metabolism,
653 $aMuscle
653 $aResidual feed intake
700 1 $aBUARQUE, V. L. M.
700 1 $aSILVA, J.
700 1 $aPOLETI, M. D.
700 1 $aBARBOSA, L. C. G. S.
700 1 $aHIGUERA-PADILLA, A.
700 1 $aGOMEZ, J. F. M.
700 1 $aCOLNAGO, L. A.
700 1 $aGERRARD, D. E.
700 1 $aSARAN NETO, A.
700 1 $aSILVA, S. L.
773 $tAnimal Feed Science and Technology$gv. 271, 114757, 2021.
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